Quickchange Primer Design Helper

Nucleotide sequence:
Substitution (mutation) positions:

Advanced Options (...give it a try first before tweaking these):
Maximum primer length: Minimum melting temperature (Tm):
Minimum % GC (Stratagene[tm] style primers only):
Minimum bases to the left (5') of the mutation: Minimum bases to the right (3') of the mutation:
Minimum/maximum overlap for Zheng et al offset style primers:/
Generate Zheng et al offset style primers
Generate Stratagene[tm] style complementary primers